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Cisplatin-induced hearing loss is mainly caused by inflammation

Initial inhibitor screens were conducted in 200 l volumes in 96 well plates with 50 M inhibitor, 100 M NADPH, and 5 nM TGR in 0.1 M potassium phosphate (pH 7.4), 10 mM EDTA with a preincubation step of 15 min

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However, an equimolar dose of dithiothreitol failed to increase GSH levels and protect mucosa from TNBS+ethanol-induced injury