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[56] Another approach, which allows to measure the glutathione redox potential at a high spatial and temporal resolution in living cells is based on redox imaging using the redox-sensitive green fluorescent protein (roGFP) [57] or redox sensitive yellow fluorescent protein (rxYFP) [58] See also Glutathione synthetase deficiency Ophthalmic acid roGFP, a tool to measure the cellular glutathione redox potential Glutathione-ascorbate cycle Bacterial glutathione transferase Thioredoxin Cysteine-containing small proteins with very similar functions as reducing agents References ^ a b Merck Index , 11th Edition, 4369 ^ Pompella, A

Archivos de medicina 2009;5(1:5)

153 StreitW

GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating