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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min

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While islet blood flow is innately required for and coupled to insulin sensing and release, extensive vasculature also makes the beta-cell uniquely poised for interactions with the immune system

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