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As we previously discussed, the GSH/GSSG ratio changes depending on the organelle and cell compartment being usually the mitochondria matrix, the space with the higher ratio in the cell (Stallons et al

For example, gene therapy has been used to reduce P-gp expression at the BBB, and RNA interference (RNAi) technology has been employed to silence the P-gp gene [86]

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However, this process depends on several cofactors, including vitamin C, iron, and B vitamins

After washing three times with TBS, the sections were stained for 5 min at room temperature with DAPI Nucleic Acid Stain (PA-3013, Lonza, Basel, Switzerland) and washed with TBS