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Open-channel probability (NPo) was calculated as: NPo= (A 1 + 2A 2 + 3A 3 + + NA N )/(A 0 + A 1 + A 2 + A 3 + +A N ) where A 0 is the area under the curve of amplitude histograms corresponding to current in the closed state, and A 1 A N represents the histograms area reflecting the different open-state current levels for 1 to N channels present in the patch