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Double staining was performed: 2 g/ml Hoechst (Sigma-Aldrich, St

However, due to the hydrolysis equilibrium inherent in polysulfide species, the use of strong electrophilic alkylating agents, including monobromobimane, led to the acceleration of polysulfide degradation through hydrolysis, thereby compromising the precision of quantification [32]

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In addition, ascorbate has the capacity to reduce free redox-active transition metal ions, such as copper and iron

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