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FIGURE 1 FIGURE 2 Individual-centric spheroids did not show cell over proliferation in long-term cell culture conditions The use of immortalized cell lines such as HepG2 or Huh7 to generate spheroids for DILI assessment often suffers from excessive uncontrolled cell proliferation resulting in enlarged spheroids with a necrotic core that can alter drug penetration and drug responses and thereby reducing the physiological relevance and predictive accuracy of the model

With 18 FGF ligands and four FGFRs, even with a single FGFR expressed, different FGFs can trigger dramatically different quantitative and qualitative responses

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