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Our in vitro and in vivo analyses supported that the tumor retention time of PpIX was significantly prolonged in A375, 4T1, and U87MG tumors after application of the MN-CCPCA patch, and the FL intensity of PpIX increased throughout the period compared with i.v

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Such improvements in the biosynthetic kinetics of PpIX not only ensure tumor oxyhemoglobin saturation (sO 2 ) and in vivo PpIX metabolic kinetics to be monitored through fluorescence (FL)/photoacoustic (PA) duplex real-time imaging, but also provide the selectivity of irradiation parameters by allowing multiple irradiations under the single topical administration of the MN-CCPCA patch

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