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Access this article Similar content being viewed by others References American Heart Association

Tissue collection Mice from each treatment group (46 animals) were euthanized through an overdose of sodium pentobarbital (300 mg/kg, Euthasol) via IP injection, followed by transcardial perfusion with 0.1 M PBS, then 4% paraformaldehyde (PFA) for tissue fixation

This suggests a possible positive selection in neoplastic cells for mutated mitochondrial genes, in keeping with observations that an aberrant mitochondrial respiration confers adaptive advantages to neurofibroma cells