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Levels of KL-6 (a marker for AEC injury), ColIV1, and ColIV3 (markers for BM destruction) were significantly elevated in the serum of animals in the induced group, signifying that BLM caused AEC damage that resulted in the release of KL-6 23 and recruitment of inflammatory cells (e.g., monocytes) leading to the release of MMPs which results in further degradation of the surrounding BM and the release of collagen type IV fragments into the blood circulation 58,64

Adherent cells were cultured for seven days in the presence of 10 ng/ml macrophage colony stimulating factor (Miltenyl Biotec, San Diego, CA) in RPMI 1640 with 10% FBS, 5% human AB serum, 1% P/S, and 10 mM HEPES to differentiate the cells into macrophages

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