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Virus-containing supernatants were ultracentrifuged for 2 h at 20,000 x g at 4 C (Hitachi CP100NX ultracentrifuge) and the pellet was aliquoted in 1 x PBS (200 l for viruses produced from one 15 cm plate dish)

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Numerous studies have proposed that GHK-Cu acts as a potential SIRT1 activator, thereby highlighting its multifaceted role in cellular processes, especially its capacity to enhance SIRT1 activity ( in vivo and in vitro experiments

Pharm Pharmacol Commun 6(11):517520 Panda S, Kar A (2001) Inhibition of T3 production in levothyroxine-treated female mice by the root extract of Convolvulus pluricaulis