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Description

All the cell lines were maintained at 37C in Dulbecco's MEM: Ham's nutrient mixture (F12) 1 : 1 (Gibco Biocult, UK) supplemented with 10% foetal calf serum, 50 IU ml 1 penicillin, 50 g ml 1 streptomycin solution, 2 mM L-glutamine, 1 g hydrocortisone and 12.5 ml HEPES buffer (1 M), all from Gibco Biocult, UK