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Examples of peptide tags include AviTag, a peptide allowing biotinylation by the enzyme BirA and so the protein can be isolated by streptavidin (GLNDIFEAQKIEWHE) Calmodulin-tag, a peptide bound by the protein calmodulin (KRRWKKNFIAVSAANRFKKISSSGAL) FLAG-tag, a peptide recognized by an antibody (DYKDDDDK) HA-tag, a peptide recognized by an antibody (YPYDVPDYA) His-tag, 5-10 histidines bound by a nickel or cobalt chelate (HHHHHH) Myc-tag, a short peptide recognized by an antibody (EQKLISEEDL) S-tag (KETAAAKFERQHMDS) SBP-tag, a peptide which binds to streptavidin (MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP) Softag 1, for mammalian expression (SLAELLNAGLGGS) Softag 3, for prokaryotic expression (TQDPSRVG) V5 tag, a peptide recognized by an antibody (GKPIPNPLLGLDST) Xpress tag (DLYDDDDK) Examples of protein tags include BCCP (Biotin Carboxyl Carrier Protein), a protein domain recognized by streptavidin Glutathione-S-transferase-tag, a protein which binds to immobilized glutathione Green fluorescent protein-tag, a protein which is spontaneously fluorescent and can be bound by nanobodies Maltose binding protein-tag, a protein which binds to amylose agarose Nus-tag Strep-tag, a peptide which binds to streptavidin or the modified streptavidin called streptactin (Strep-tag II: WSHPQFEK) Thioredoxin-tag

Pubmed.gov

Reagents, including the inhibitors and mouse lines, can be requested from the corresponding author and will be distributed upon reasonable request and availability to fill all requests

Goss 1 , Abbas Rana 1 , Fasiha Kanwal 1 , Gene Y

Esta inestabilidad limita su utilidad, tanto en trminos de los productos en los que estn incorporados como de la variedad y el alcance de los productos en los que podran incorporarse si no fuera por su inestabilidad