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Mercaptoacetic acid (19.82 g, 215.15 mmol) and trifluoroacetic acid (TFA, 100 L) were dissolved in acetone (5.00 g, 86.09 mmol)

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Supporting this notion, we showed that in rat cerebral cortical cultures, short term (48 h) proteasome inhibition results in the accumulation of soluble Ub-proteins without significant loss in neuronal viability (Metcalfe et al., 2012)