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The prepared lysates were diluted 1:5 in an assay buffer and fluorescence intensities were measured using a Varioskan Flash spectral scanning multimode reader (Thermo Fisher Scientific, Inc.)

Third, this was a single-center study with a relatively small sample size, thus, requiring validation through larger-scale multicenter studies in the future

436 Selinexor effectively impedes the nuclearcytoplasmic transport of the SARS-CoV-2 nuclear protein, thereby exerting inhibitory effects on viral proliferation