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Bacteria were then incubated with freshly isolated human neutrophils (7.5 10 4 ), that were purified from blood of healthy donors by the Ficoll/Histopaque density gradient method 73 , for another 15 min at 37 C with shaking

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Randomization was done using computer generated random sequence allocation in a 1:1 ratio to either receive intravenous rtPA and intravenous N Acetyl Cysteine or IV rtPA alone