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Span 60, with its long alkyl chain (C18), lowers surface tension, facilitating smaller vesicle formation, while also contributing to bilayer rigidity, thus stabilizing smaller, uniform particles (Figure 2

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Interestingly, the MK2206 effect on the NADPH/NADH ratio was less pronounced in the unirradiated AKT-E17K cells compared to the AKT-WT cells ( Figures S3E )