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The journals of gerontology Series A, Biological sciences and medical sciences

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After SDS-PAGE analysis on a 15% gel under reducing conditions, the fraction containing the majority of the recombinant proteins was subjected to size-exclusion chromatography by loading the protein extract on an Ultrogel ACA44 (5 75 cm, Biosepra) column equilibrated with a 30 mM Tris-HCl pH 8.0, 1 mM EDTA, and 200 mM NaCl buffer