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When endothelial NOS function is abnormal caused by pathological stimulating factors such as insulin resistance, impaired glucose tolerance and obesity, its catalytic process becomes "uncoupled," leading to the production of superoxide anion (O) instead of NO

Although horses can synthesize some choline endogenously, requirements may increase under metabolic stress, high performance demands, or when diets are low in methyl donors such as methionine or folate

The generation of GSNO can serve as a stable and mobile NO pool which can effectively transduce NO signaling