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The amphiphilic cell-penetrating peptide (CPP) exhibits potential advantages in terms of enhanced penetration through the skins lipid barrier and efficient transportation of GHK to the cellular surface, thereby demonstrating a remarkable efficacy in skin regeneration

H1299 cells were obtained from ATCC and were maintained in McCoys (Gibco) containing 10% FBS (Gibco) and 1% Antibiotic-Antimycotic (Gibco)

Nat Commun 15 , 7294 (2024)

If the mechanism is fairly focused but the dosing schedule is poorly chosen, the observed effects may reflect protocol strain, cumulative exposure patterns, or handling inconsistencies rather than the peptides intended pharmacology

HT-29 and DLD-1 cells were then selected with puromycin (Sigma-Aldrich) for 3 weeks and analysed for loss of PHGDH expression