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After centrifugation at 4075 g for 10 min at 4 C and filtration through a 0.45-m pore filter, the supernatant was purified using chromatography using anti-Flag (M2) antibody-conjugated affinity beads (Sigma-Aldrich) for STAND proteins or anti-Myc antibody-conjugated affinity beads (MBL) for Myc-Y13-259

We hypothesized that increased oxidative stress and reactive oxygen species (ROS) levels, similar to Gb3 accumulation, are involved in renal remodeling in Fabry disease 22,23,24,25,26,27,28

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