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The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea

Einige Lebensmittel enthalten natrlicherweise Glutathion, das den Glutathion-Stoffwechsel im Krper untersttzen kann

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

Similarly, Rajegowda et al

The main astrocytic reactions that consume ATP are shown in Fig