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Our insulin sensitivity goes down leading us towards more fat gain

The supernatant was then collected and protein was assayed on 96-well plates by adding 3 L of standard and 150 L of protein assay dye reagent (Bio-Rad, Hercules, CA, USA) diluted 1:5 with distilled water

While these results are suggestive of ferroptosis in this pathological scenario, further work using ferroptosis-specific inhibitors such as Fer-1 will be helpful to confirm this point