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2.1 The discovery and fundamental functions of NOX enzymes The classical nicotinamide adenine dinucleotide phosphate (NADPH) oxidase was first identified in neutrophils in 1933, when Baldridge and Gerrard observed a significant increase in oxygen consumption during bacterial phagocytosis (Cross and Segal, 2004)

These findings, although obtained from models of oxidative stress and mitochondrial dysfunction rather than in autism models, are highly relevant because they target biological mechanism that are also characteristic of ASD (Sachdeva et al., 2022)

12 Discussion To date, there are few studies demonstrating utility of topical glutathione in treating hyperpigmentation, oxidative damage, TEWL, and improving skin elasticity