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Synthesis and characterization of poly (3-hydroxybutyrate)/chitosan-graft poly (acrylic acid) conjugate hyaluronate for targeted delivery of methotrexate drug to colon cancer cells

Taken together, current evidence indicates that T cells in RA patients exhibit a distinct metabolic signature characterized by dysregulated glycolysis and impaired mitochondrial function

Lencer (Boston Childrens Hospital, Harvard Medical School and Harvard Digestive Diseases center, United States) were cultured in MCDB 131 medium (Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco), 2 mM l-glutamine (Gibco), 25 g/mL streptomycin (Gibco), 25 U/mL penicillin (Gibco), 10 ng/mL recombinant mouse epidermal growth factor (Gibco), and 1 g/mL hydrocortisone (Sigma-Aldrich), at 37 C in a humidified atmosphere of 5% CO 2

Use within 2-3 weeks

The minimum sample intake is 5 g