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Ambion/Life Technologies (Grand Island, NY, USA)) were transfected using lipofectamine 2000 in OPTIMEM according to the manufacturers instructions

Sublingual oral sprays are designed to begin absorption through the tissues under the tongue before the remaining liquid is swallowed, offering a convenient alternative to conventional oral tablets

Modulation of tau phosphorylation by environmental copper

For the reconstitution experiment, silent point mutations were introduced into the ABCC1 cDNA using PCR mutagenesis to avoid targeting by ABCC1 sgRNAs and the product was cloned into a pMSCV-PGK-BlastR retroviral expression vector using Gateway cloning

Moreover, GPX4 degradation can also result in ferroptotic cell death