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mTORC1 inhibition of AMPK A novel lysosomal 2-AMPK activation pathway A 2015 report from the Petersen lab showing nitrogen stress elicits TORC1 inhibition and activation loop (T189) phosphorylation of the AMPK fission yeast homolog Ssp2, independent of overt changes to adenine nucleotides [84], was the catalyst for the hypothesis that mTORC1 exerts direct control over AMPK

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Compounds of oligomycin (10 M), carbonyl cyanide m-chlorophenyl hydrazone (40 M), and rotenone (5 M) were prepared and loaded into XFe24 sensor cartridges from port A to C for sequential injection over time