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The heart was then cut down, frozen for 2 h, and cut into eight slices using a scalpel blade and stained in a Na 2 HPO 4 /NaH 2 PO 4 solution (2:1 ratio of 0.1 M stock solutions until pH 7.4) with 10 mg/mL 2,3,5-triphenyltetrazolium chloride (TTC) (Sigma-Aldrich) to identify the area at risk (AAR) and infarcted area (IA, white in appearance), respectively

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What is also unknown is the effect on cognition, and any neuroprotective benefit, in younger or healthy individuals remains unknown, some positive results have been reported, 6 however a large scale confirmatory study is still required

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