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Regulatory Hurdles 3.2.2

Feedback from stakeholders via the WADA Health, Medical, and Research Committee (HMR)

To perform Co-IP of endogenous SORLA from brain tissues, mouse cortices were dissected and homogenized on ice using an ice-cold IP buffer containing 10 mM Tris (pH 7.6), 10% glycerol, 0.5% Nonidet P-40, 5 mM EDTA, 5 mM EGTA, and protease inhibitors (Roche Diagnostics)