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Prior studies have used the cell cycle proteins p16, p21, p53 and Rb to differentiate between dividing and non-dividing cells, but the non-dividing cells can include senescent cells and quiescent cells 36

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Parental MCF-7 were cultured in growth medium containing 5% fetal bovine serum (FBS) with increasing amount of Tamoxifen, starting from 0.5 M initially, finally up to 5 M in a period of around 6 months

doi: 10.1038/s41598-018-27580-1 90

In 40% of the subjects, treatment with fish oil significantly reduced bronchoconstriction (as measured by im- proved forced expiratory volume for 1 sec (FEV1) and forced expiratory flow 2575% (FEF2575)