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The human CPT1A gene (Gene ID: 1374, EC 2.3.1.21), located at chromosomal band 11q13.3 and spanning 23 exons, encodes the liver isoform

Our SOP builds on these foundations by integrating these elements into a unified, stepwise framework adapted from pediatric protocols specifically for the adult ICU context

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All patient primary diagnosis slides were reviewed by a single pathologist (E.E.) to confirm primary epithelial ovarian cancer diagnosis

Control cells were cultured with growth medium consisting of DMEM (1 g/L glucose, Thermo Fisher Scientific), 10% FBS, and 1% antibiotics