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303 Additionally, inhibition of mitochondrial respiration failed to rescue the ES/Cu toxicity, 303 and intracellular copper can be released from the ES/Cu complex and become bioavailable outside the mitochondria, suggesting a great likelihood of mitochondrial-independent cuproptosis

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Pharmacol Ther 244:108373

10.1016/j.bbrc.2016.10.086 Biochem Biophys Res Commun

However, one study presented conflicting results in mice, reporting a current-dependent increase in the firing rates of L5p following DOI application (2050 nA) (De Gregorio et al., 2021)