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Buffer II (PBS, pH 7.4, 0.1% bovine serum albumin, 2 mM EDTA) was utilized to wash the pellets twice for removing residual cytosolic GFP-LC3B present in the pellets

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Image acquisition Scans were conducted at the Sir Peter Mansfield Imaging Centre, University of Nottingham, using a 7 Tesla Philips Achieva scanner (Philips Medical Systems, Best, The Netherlands), a volume transmit head coil, and a 32-channel receive head coil