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Description

Cloning of ChaC1 constructs in pRS416 vectors For the construction of ChaC1 under ADH1 and CYC1 promoters, the TEF-ChaC1 construct was digested with XbaI and SacI enzymes and replaced with ADH1 and CYC1 promoters from p416ADH and p416CYC plasmids respectively

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Acid-catalyzed transesterification is typically conducted under high-temperature, high-pressure conditions to accelerate the reaction rate and improve yield

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PMID: 229 GhalandarlakiNAlizadehAMAshkani-EsfahaniS

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