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10.3390/nu15234852 Nutrients 103 PalmerJ

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Antioxidant kinetics in lung lavage fluid following exposure of humans to nitrogen dioxide

2.13 DHE stain For human umbilical vein endothelial cells (HUVECs), after different treatment groups, the cells were cultured in a glucose-free medium under hypoxic conditions for 3 h

Each sample was then split into two fraction (100 L) and treated as follows: For H 2 S measurement, 100 L of each sample was precipitated with 1 mL of absolute MeOH at 40 C for 2 h, centrifuged (16,000 g at 0 C), and obtained supernatant was evaporated and the residue was resuspended and sonicated in 100 L of 50 mM HEPES buffer (pH 7.4)