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After incubation at ambient temperature for 1 h, the mixture was added with 100 L 0.1% N,N-dimethyl-p-phenylenediamine (DMPD, D4139, Sigma-Aldrich) in 5 M HCl and 100 L of 20 mM FeCl 3 in 6 M HCl

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Furthermore, a perceived flaw of the older system was that although 6 was the lowest score assigned to a tumour in practice, a score of 6 out of 10 nevertheless might contribute to patient fear by implying an intermediate rather than an excellent prognosis