a conservative change such as a hydrophobic residue to another hydrophobic residue, or a non-conservative change, e.g., a hydrophobic residue to Modifications in the test polypeptide can be introduced by, e.g., error-prone PCR, shuffling, oligonucleotide-directed mutagenesis, assembly PCR, sexual PCR mutagenesis, in vivo mutagenesis, cassette mutagenesis, recursive ensemble mutagenesis, exponential ensemble mutagenesis, site-specific mutagenesis, gene reassembly, gene site saturated mutagenesis (GSSM), synthetic ligation reassembly (SLR) and/or a combination thereof
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Additionally, analyzing the expression levels of peroxisomal and chaperone-related markers in infected animal tissues would help validate the functional significance of the GRA23-PEX3 and GRA23-TRAP1 interactions in vivo
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As the BHT + AD group, the animals received 3 mg streptozotocin per kg body weight by stereotactic intraventricular injection
