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Acknowledgements This work was supported by Chang Gung Memorial Hospital [CMRPG391311, CMRPG3A1131, CMRPG3H0611 and Grants CIRPD1D0071-3

J.BarrotM.DiLeoneR

weeks 5 to 8 use 1.5 mg or 9 units

Following transfection, the cells were lysed with reporter lysis buffer (Promega), and luciferase activity was measured with the Dual-Luciferase Reporter Assay System (Promega, E1910) according to the manufacturer's protocol and quantified using a GloMax 20/20 Luminometer (Promega, USA)