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Attenuation by time-restricted feeding of high-fat and high-fructose diet-induced NASH in mice is related to Per2 and ferroptosis

These systems operate in parallel but influence many of the same biological pathways

The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS

Protocol steps included: equilibra- tion, sample capture, wash, elution

Focsan AL, Polyakov NE, Kispert LD (2017) Photoprotection of Haematococcus pluvialis algae by astaxanthin: unique properties of astaxanthin deduced by EPR, optical and electrochemical studies