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The cells were washed twice with PBS, trypsinized, and then stained with propidium iodide(1 mg/mL)

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Similarly, HHcy induced by either a folate-deficient diet or by directly infusing Hcy into the mouse brain worsens the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mediated PD symptoms such as dopamine depletion, substantia nigra pars compacta neuronal degeneration and motor dysfunction [82]