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Since the nocturnal concentration of melatonin is approximately 310 times higher than the daily one (Odaci and Kaplan, 2009), the nocturnal application of the exogenous melatonin would be reinforced by the physiological peak of melatonin at night, thus leading to enhanced effects on regeneration according to Rateb et al

That firmness with its gentle check may quell Passions that heave the breast with angry swell, Darken the eye, and fiercely knit the brow, And would convert lifes cup to one of woe, Unless to wise restraint now made to yield

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International Journal of Molecular Sciences 2024, Vol 25, Page 124 [Internet]

Finkemeier and Sweetlove, 2009