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Equal amounts of cell protein lysates were separated by SDS PAGE and transferred to PVDF membranes (Millipore, Darmstadt, Germany)

Since no other cause for the metabolic acidosis could be found, the possibility of accumulation of 5-oxoproline by paracetamol and flucloxacillin was recognised and the treatment with paracetamol was stopped

Different substrates can be used, such as p-nitrophenyl phosphate (p-NPP) or 5-bromo-4-chloro-3-indolyl phosphate (BCIP) (78,79)

Purchase from reputable sources, ensure proper refrigeration, and cook thoroughly to an internal temperature of at least 70C to eliminate potential pathogens