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The genetic association between the genes encoding methionine synthase and N5,10-methylenetetrahydrofolate reductase suggests a close relationship between these enzymes and the tetrahydrofolate metabolism (Figure 2) [14, 15, 16, 17]

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J Neural Transmission

Starling, J

Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h