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The sections were first washed with PBS and then permeabilized with 0.1% Triton X-100 (#T9284, Merck, Germany-Aldrich) in PBS for 10 min at room temperature

HA-HMOX2 was immunoprecipitated using an anti-HA antibody and SureBeads Protein G magnetic beads (161-4023, Bio-Rad Laboratories)

Conclusions MPs/NPs, as pervasive environmental contaminants, infiltrate humans through multiple exposure routes, traverse biological barriers, and accumulate in the central nervous systemconstituting a novel environmental hazard for PD pathogenesis