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Furthermore, our single-cell mass cytometry approach was limited to ~40 proteins, capping the number of metabolic pathways we could interrogate simultaneously

The study planned to include 200 patients with type 2 diabetes who were not on any antidiabetic drugs in the 90 days prior to Visit 1 (except in certain clinical situations, such as acute illness, hospitalization, or elective surgery, short-term (14 days) use of insulin)

Antibodies were diluted in a blocking solution

These findings suggest that the influence of estrogen on the inflammatory response induced by CIH may be concentration-dependent, warranting further investigation to determine whether similar concentration-dependent effects of estrogen are observed in other tissues

Collodel G, Moretti E, Noto D, Iacoponi F, Signorini C