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Formulated LNP has a median particle diameter of about 60-70nm, as measured by nanoparticle tracking analysis (ZetaView, particle Metrix), andsiRNA encapsulation efficiency of about 80-90%, e.g., by modified Quant-iT TM On day 6 of culture, M2 macrophages were polarized using 20ng/ml human IL-10(Biolegend, San Diego, Calif.)