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10.3390/cancers14246203 10 AyusoJ

The resulting construct was biolistically transformed into the WT strain as described previously 54 , and positive transformants were selected on hygromycin (200 g ml -1 ) and confirmed via PCR and Southern blot analysis

The in vitro experiments have confirmed that vitamin D could inhibit the growth of Mtb and secretion of pro-inflammatory cytokines in peripheral blood monocytes (Martineau et al., 2007), stimulate the receptor-induced expression of antimicrobial peptides, and up-regulate LL37 gene to induce the autophagy-related proteins Atg5 and Beclin-1 to mediate the killing effect (Periyasamy et al., 2020)

Cusick SE, Georgieff MK