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Earlier studies indicated that isocitrate lyase is required for the persistence of Mycobacterium tuberculosis in lungs in tail-vein injected mice ( BacA mutants of Mycobacterium tuberculosis lose their capacity to maintain chronic persistence in tissue of murine model infected via aerosols ( The disappearance of mutants from calf tissue was in agreement with the results of an ex-vivo viability assay, where wild-type MAP survival was also hindered by effector immune responses in bovine macrophages alone and macrophages incubated with unprimed PBMCs in both vaccine groups