melanotes

melanotes The Johns Hopkins (@jhumelanotes) • Facebook Variety Sticks 4 liters -

Name: melanotes The Johns Hopkins (@jhumelanotes) • Facebook Variety Sticks 4 liters -
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Antimicrobial resistance: a growing serious threat for global public health

Nature 473 , 308316 (2011)

For the Pho860 assay, 1 OD 600 units of yeast cells were harvested, resuspended in 300 L Pho860 lysis buffer (20 mM PIPES-KOH, pH 6.8, 50 mM KCl, 100 mM KOAc, 10 mM MgSO 4 , 10 M ZnSO 4 , 0.5% Triton X-100), followed by 5 min of vigorous mixing by vortex at 4 C