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Organ suspensions were subjected to serial 3-, 5- or 6-fold dilutions (depending on the state of disease progression) in modified Schneides Drosophila medium (Leitherer et al., 2017), with 12 replicates per dilution step

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References 1.Bulavenko O., et al

Unlike vitamin C, vitamin E, and polyphenols that indiscriminately neutralize all reactive species (including beneficial signaling molecules), molecular hydrogen targets only the most damaging oxidants like hydroxyl radicals while preserving normal redox signaling